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Today we're walking through the Excalibur casino in Las Vegas, starting from inside the parking garage and working our way through the entire property. Coming out of the parking garage, we begin with a quick look over the fence at the Excalibur swimming pool, then we take in the castle-style exterior of the hotel towers and turrets. As we enter inside, we see the whimsical, medieval architecture and pass through various areas of the casino starting with the sports book and the TAP Sports Bar. Walking through the casino floor and slot machines, we take another peek at the Sprinkles cupcake vending machine, and we venture inside Johnny Rockets restaurant where a burger and fries now costs $22.95, a $1 increase from when we last walked through Excalibur. We pass the hotel lobby and the entrance from the Strip (with a moving sidewalk) before heading downstairs to the arcade area, where Dairy Queen and carnival games typically draw a younger crowd. After a loop through the lower level, we take the escalator back up to the casino and continue our walkthrough. We pass Dick's Last Resort, where restaurant patrons are playfully insulted, and we stroll past rows of slot machines, decorative knight armor, and a mostly unused lounge that's open only 16 hours per week. Taking an escalator up a floor, we check out Buca di Beppo and the Excalibur buffet, which is open as it's during brunch hours. Finally, we follow the indoor walkway toward the Luxor, where the moving walkways have been removed but the stained middle carpet remains, and we end with a look at the food court. If you're curious what the Excalibur casino looks like in 2025 or just want a relaxed, first person walkthrough of one of the more playful themed resorts on the Strip, this video gives you a complete look. Thanks for watching. I appreciate you being here! 00:00 Excalibur Casino Parking Garage 00:34 Excalibur Swimming Pool 00:56 Excalibur Casino Exterior In Daytime 02:06 Entering The Casino 02:54 Castle Style Architecture 03:27 Sports Book / Convention Chairs 04:00 TAP Sports Bar 04:32 Excalibur Casino Walkthrough 05:10 Sprinkles Cupcake Vending Machine 05:41 Johnny Rockets $22.95 Burger & Fries 06:41 Casino Walkthru 07:23 Excalibur Hotel Lobby 08:12 Entrance From Las Vegas Blvd / Moving Sidewalk 09:41 Downstairs Excalibur Arcade Area / Dairy Queen 11:25 Carnival-Type Games 12:29 Escalators Up To Casino Floor 13:33 Excalibur Casino Walk 14:34 Dick's Last Resort Restaurant 15:20 Slot Machines 16:03 Knight's Armor / Castle Architecture 17:03 Closed Lounge (Only Open 16 Hours Per Week) 17:40 Heading Upstairs 18:50 Buca Di Beppo Family Style Restaurant 19:49 The Buffet At Excalibur (Open) 21:01 Walkway To Luxor (Removed Moving Walkways) 23:37 Vegas Tourist Experience Thoughts 24:48 Excalibur Food Court 26:52 Construction Wall Covering Starbucks Location 27:04 Del Taco $4.99 Taco #ExcaliburCasino #LasVegas2025 #VegasWalkthrough #CasinoTour #LasVegasStrip #VegasHotelTour #VegasCasinos #FirstPersonWalk #SlotMachines #VegasTravel #4KWalk

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En ésta ocasión, nos salimos un poco de la norma y os traigo un blog de cómo fue mi experiencia junto a mis amigos en el salón del manga de Barcelona. (Acabé endeudado y me embargaron el piso). Twitter: https://twitter.com/guishermos?t=8FNOTfLJDViByDag1wH1nA&s=09 Instagram: https://www.instagram.com/asturianosincomplejos?igshid=YzVkODRmOTdmMw==

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#dotblot #RohitMane #ScientistRacademy Dot Blot is a cheaper, easier and faster technique to detect the presence of Proteins and Nucleic Acids in a biological sample. Due to the simplicity of the technique it widely used as a ideal diagnostic tool. This post covers the Protein Dot Blot Technique. Dot Blot Principle Dot Blot works based on the immunodetection principle for identifying specific protein. Dot Blot - PrincipleDot Blot - Technique: Overview Proteins are immobilized on to a protein binding membrane, PVDF or Nitrocellulose. After Protein Immobilization membrane is blocked to avoid non-specific binding, using blocking buffer. Immobilized protein is then incubated with a primary antibody (for 30mins to 1 hour). The antibody is specific to the protein and is visualized either with a specific tag or with a secondary antibody.  The secondary antibody generally will carry a tag that allow the visualization of protein. Blots can be visualized using methods such as Chemiluminescence or Fluorescence. Common Tags used in Dot Blots are enzymes conjugated to the secondary antibody. Upon addition of substrate, enzyme acts on the substrate to develop colour. Commonly preferred enzymes are HRP(Horse Radish Peroxidase) and Alkaline Phosphatase (AP) Advantages of Dot Blot over Western Blot Non-Electrophoresis Technique: Dot Blot is non-electrophoresis technique, means that one need not do protein electrophoresis. Proteins can directly immobilized on to the membrane. Simple & Easy Method: No messy work of PAGE run and gel to membrane transfers, proteins are directly immobilized on membrane, making this method much easier and faster. Time: It is a faster method, takes ~3hours, whereas western blot may even take up to 2 days. Including the protein electrophoresis and gel to membrane transfer optimization. Dot Blot is not as informative as Western Blot, but it is inexpesive, quick and easy method for the simple protein detection.  dot blot technique by shomus biology dot blot technique in hindi

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